Product Name :
Phospho-Smad2 (S255) Recombinant Rabbit Monoclonal Antibody [JF0882]
Predicted band size :
52 kDa
Observed band size :
60 kDa
Synonyms:
Drosophila, homolog of, MADR2 antibody hMAD-2 antibody HsMAD2 antibody JV18 antibody JV18-1 antibody JV181 antibody MAD antibody MAD homolog 2 antibody MAD Related Protein 2 antibody Mad-related protein 2 antibody 展开
Function :
Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser255 of Human Smad2 aa 229-272 / 467.Iridium(III) acetate trihydrate site
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IHC-P, IP
Coupling :
unconjugated
Clone number:
JF0882
RRID:
AB_3070296
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.102879-42-5 Purity Aliquot store at -20℃ or -80℃.PMID:33413734 Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 52 kDa
Positive control :
HeLa cell lysate, HeLa treated with 200nM Calyculin A for 1 hour cell lysate, RAW264.7 cell lysate, mouse uterus tissue.