Product Name :
iNOS Rabbit Polyclonal Antibody
Predicted band size :
131 kDa
Observed band size :
131 kDa
Synonyms:
HEP-NOS antibody Hepatocyte NOS antibody HEPNOS antibody inducible antibody Inducible nitric oxide synthase antibody Inducible NO synthase antibody Inducible NOS antibody iNOS antibody MAC NOS antibody Macrophage NOS antibody 展开
Function :
Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.
Antibody Type:
Rabbit Polyclonal Antibody
Immunogen :
Synthetic peptide within human iNOS aa 1,104-1,153 / 1,153.
Species reactivity:
Human, Mouse, Rat, Chicken
Verify application:
IF-Cell, FC, WB, IHC-Fr
Coupling :
unconjugated
Clone number:
RRID:
AB_3069098
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze/thaw cycles.Formula of 19715-49-2
Storage buffer solution:
1*PBS (pH7.Formula of 1310680-18-2 4), 0.PMID:33557640 2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Immunogen affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 131 kDa
Positive control :
RAW264.7 treated with 1μg/mL LPS for 24 hours whole cell lysate, RAW264.7 whole cell lysate, HeLa cell lysate, A549 cell lysate, A549, LOVO.