Product Name :
Phospho-AKT (S473) Recombinant Rabbit Monoclonal Antibody [PSH04-44]
Predicted band size :
56 kDa
Observed band size :
56 kDa
Synonyms:
AKT1 antibody AKT1_HUMAN antibody MGC99656 antibody PKB antibody PKB-ALPHA antibody PRKBA antibody Protein Kinase B Alpha antibody Protein kinase B antibody Proto-oncogene c-Akt antibody RAC Alpha antibody 展开
Function :
RAC(Rho family)-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This enzyme belongs to the AKT subfamily of serine/threonine kinases that contain SH2 (Src homology 2-like) protein domains. It is commonly referred to as PKB, or by both names as “Akt/PKB”. The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1.66937-72-2 Chemscene It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Phospho-Akt (Ser473) [PSH04-44] Recombinant Rabbit mAb detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser473 of human Akt1.1-Bromo-2-fluorobenzene site
Species reactivity:
Human, Mouse, Rat
Verify application:
WB
Coupling :
unconjugated
Clone number:
PSH04-44
RRID:
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.PMID:33533261 Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer solution:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 56 kDa
Positive control :
HEK-293 cell lysate, C6 cell lysate, C6 treated with 100ng/mL PDGF for 5 minutes cell lysate, MCF7 cell lysate, MCF7 treated with 100nM Calyculin A for 30 minutes cell lysate, SH-SY5Y cell lysate, SH-SY5Y treated with 100ng/mL PDGF for 5 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 100ng/mL PDGF for 1 hour cell lysate.