Y weight), metformin (20 mg/kg physique weight) and atorvastatin (five mg/kg physique weight).Determination of glucose and cholesterol levels and total antioxidant[11,12]By comparing its spectral information using the published data,[17] compound two was identified as lupeol3acetate [Figure 2]. The identification was also confirmed by melting point and cochromatography with an authentic sample. This compound was previously isolated in the leaves from the same plant.[1]CompoundNMR spectrum displayed the characteristic signals of a caffeic acid and quinic acid moieties.[18] The downfield shift of H4 (H 5.08) indicates acylation in the quinic acid at C4 hydroxyl group. This was confirmed by the downfield shift of C4 to C 79.61. By comparing the spectral information of this compound together with the published information,[18] compound three was identified as 4caffeoylquinic acid (cryptochlorogenic acid) [Figure 3].132182-92-4 custom synthesis This compound was isolated for the initial time in the plant.Biological studyBlood samples were taken in the 6th week of experiment then centrifuged at 4000 rpm for ten minutes. The supernatant (serum) was separated and divided into three portions. Serum glucose, [13] cholesterolPharmacognosy Research | AprilJune 2013 | Vol 5 | IssueThe aqueous extract of the fruits showed significant antihyperglycemic, hypocholesterolemic and a potent antioxidant effect in the model group. Water soluble polyphenolic compounds mainly tannins and flavonoids,Fayek, et al.: New triterpenoid acyl derivatives and Manilkara zapotaTable 1: Antioxidant, antidiabetic and hypocholesterolemic activities of M. zapota fruitsGroups Unfavorable manage Model of diabetic and hypercholesterolemic non treated rats Model treated with reference drugs Model treated with alcoholic extract Control group treated with alcoholic extract Model treated with aqueous extract Control group treated with aqueous extract Model treated with aqueous homogenate Manage group treated with aqueous homogenateaTAO (mM/L) (Mean D) 0.Formula of 2322869-99-6 309.PMID:33449821 033 0.127.011 0.601.020a 0.201.020 0.287.088 0.699.028a 0.764.269b 0.332.059a 0.447.Glucose level (mg/dl) (Imply D) 81.53.13 151.03.six 86.85.2a 136.00.15 80.0.07 90.12.6a 74.six.65 95.3.97a 74.five.Cholesterol level (mg/dl) (Mean D) 64.1.99 90.5.02 65.2a 55.five.24a 53.four.40 88.00.7a 64.9.24 82.30.8 65.8.Worth is considerable distinction when in comparison with the untreated model group at P0.05; bvalue is important difference when in comparison with the negative control group at P0.The model group treated with all the alcohol extract on the fruits showed marked decrease of cholesterol level, minimal improvement of glucose level, whilst no significant adjust in the TAO level was observed. A minimal improvement in the cholesterol level with no substantial distinction was observed inside the model group treated using the aqueous homogenate in the fruits, while substantial antihyperglycemic and antioxidant activities, reduced than that of the aqueous extract, were observed. The higher fibre content material inside the aqueous homogenate from the fruits (12.28 ),[23] may contribute to its slow digestion and absorption, this could clarify the decrease within the amount of its bioactivies when compared with the aqueous extract of the fruits.[24] The tested extracts showed no substantial adjust within the blood glucose degree of the normal rats, precisely the same because the antihyperglycemic drugs biguanides e.g., metformin.[25]Figure 1: amyrin 3(3′ dimethyl) butyrate (Compound 1)
Molecular Vision 2013; 19:20112022 http://www.molvis.org/molvis/v19/2011 Received 1 March 2013 | Accepted 24.
