E1 (Takara, Shiga, Japan) at 37uC for 15 min. To terminate the
E1 (Takara, Shiga, Japan) at 37uC for 15 min. To terminate the trypsinization, Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 (DMEM/F12) containing 10 (v/v) fetal bovine serum (FBS) was added and…